Data/hora: 11/05/2024 15:30:43

Biblioteca(s): 	Embrapa Unidades Centrais.	
Data corrente: 	29/04/2016	
Data da última atualização: 	29/04/2016	
Autoria: 	SPRADA, A. G.; ROSA, M. P. da; MACHADO, A. K.; PIPPI, N. L.; BAYARD, P.; CRUZ, I. B. M. da.	
Afiliação: 	ARÍCIA GOMES SPRADA, CCR/UFSM; MATHEUS PIPPI DA ROSA, CCR/UFSM; ALENCAR KOLINSKI MACHADO, CCS/UFSM; NEY LUIS PIPPI, CCR/UFSM; PAULO BAYARD, CCR/UFSM; IVANA BEATRICE MÂNICA DA CRUZ, CCS/UFSM.	
Título: 	Toxicity and oxidative stress of canine mesanchymal stromal cells from adipose tissue in different culture passages.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, supl. 1, p. 15-20, dez. 2015.	
Idioma: 	Inglês	
Conteúdo: 	Stem cells in regenerative therapy have received attention from researchers in recent decades. The culture of these cells allows studies about their behavior and metabolism. Thus, cell culture is the basis for cell therapy and tissue engineering researches. A major concern regarding the use of cultivated stem cell in human or veterinary clinical routine is the risk of carcinogenesis. Cellular activities require a balanced redox state. However, when there is an imbalance in this state, oxidative stress occurs. Oxidative stress contributes to cytotoxicity, which may result in cell death or genomic alterations, favoring the development of cancer cells. The aim of this study was to determine whether there are differences in the behavior of cultured mesenchymal stem cells from canine adipose tissue according to its site of collection (omentum and subcutaneous) evaluating the rate of proliferation, viability, level of oxidative stress and cytotoxicity over six passages. For this experiment, two samples of adipose tissue from subcutaneous and omentum where taken from a female dog corpse, 13 years old, Pitbull. The results showed greater levels of oxidative stress in the first and last passages of both groups, favoring cytotoxicity and cell death.	
Palavras-Chave: 	Células-tronco; Culture; Passage; Passagem; Radicais livres; Stem cell; Viabilidade celular.	
Thesagro: 	Produção vegetal.	
Thesaurus Nal: 	Cell viability; Free radicals.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/142669/1/Toxicity-and-oxidative-stress.pdf	
Registro original: 	Embrapa Unidades Centrais (AI-SEDE)	
Exemplares: 	AI-SEDE - (59412-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS